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Bacillus sp. 3 is one of symbiotic bacteria of marine sponge Haliclona fascigera obtained from Mandeh Island, Pesisir Selatan, West Sumatra that can produce antibacterial compounds. This study aims to isolate the antibacterial compounds produced by Bacillus sp.3 (A1). The methods that has been used were bacterial cultivation on Nutrient Broth medium using Shaker Incubator at 150 RPM at 37oC for 48 hours and isolation of antibacterial compound. The isolation method of the compound was carried out by column chromatography method. The testing of antibacterial activity was done by using agar diffusion method. Characterization of isolated compounds were performed using UV and IR spectrophotometer. From this research, two compounds RJ1 and RJ2 were obtained. The compound RJ1 was yellow oily (2.8 mg). The compound RJ1 can inhibit Staphylococcus aureus ATCC 25923 at a 1% of Minimum Inhibition Concentration (KHM). RJ2 compound was yellow oily (3.1 mg). The RJ2 compound can inhibit Escherichia coli ATCC 25922 at a 1% of Minimum Inhibition Concentration (MIC). ABSTRAK: Bacillus sp. 3 (A1) merupakan salah satu isolat bakteri simbion spon laut Haliclona fascigera asal Pulau Mandeh, Pesisir Selatan, Sumatra Barat yang dapat memproduksi senyawa antibakteri. Penelitian ini bertujuan untuk mengisolasi senyawa aktif sebagai antibakteri yang dihasilkan oleh Bacillus sp.3 (A1). Metoda yang digunakan yaitu kultivasi bakteri pada media Nutrient Broth menggunakan Inkubator Shaker dengan kecepatan 150 RPM pada suhu 37oC selama 48 jam dan isolasi senyawa antibakteri. Metode pemisahan senyawa dilakukan dengan metoda kromatografi kolom. Pengujian aktivitas antibakteri dlakukan dengan metoda difusi agar. Karakterisasi senyawa hasil isolasi dilakukan dengan menggunakan spektrofotometer UV dan spektrometer IR. Dari penelitian ini didapatkan dua senyawa RJ1 dan RJ2. Senyawa RJ1 berupa minyak berwarna kuning (2,8 mg). Senyawa RJ1 dapat menghambat bakteri uji Staphylococcus aureus ATCC 25923 pada Konsentrasi Hambat Minimum 1%. Senyawa RJ2 berupa minyak berwarna kuning (3,1 mg). Senyawa RJ2 dapat menghambat bakteri uji Escherichia coli ATCC 25922 pada konsentrasi hambat minimum 1%.

Isolasi dan Karakterisasi Senyawa Antibakteri dari Fraksi Etil Asetat Bakteri Bacillus sp.3 (A1) yang Bersimbiosis dengan Spon Laut Haliclona fascigera