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The sperm quality determines the success of Artificial Insemination (AI). The simple diluent, which had a buffer and isotonic characteristic, can be used as a chicken semen diluent. The purpose of this study was to compare the sperm quality of native chicken in Physiological Natrium chloride (NaCl) and Ringer’s lactate (RL) diluent at room temperature storage and to test the egg fertility based on the best storage time. This study used semen from 2 native chickens, storing semen for 2 hours at room temperature with an observation interval of 30 minutes. Insemination was carried out on 20 native chickens. The parameters observed were motility, viability, and abnormality of sperm. Statistical analysis of sperm quality using Analysis of Variant (ANOVA) nested patterns and analysis of egg fertility using the chi-square test. The results showed that the addition of 0.9% NaCl and RL diluent had a significant effect (P<0.05) on the decrease in semen quality during storage at room temperature. The best storage time at room temperature, which was still able to maintain the quality of the was at 30 minutes, and these results were used for AI applications with significantly different on egg fertility (P<0.05) respectively 82.61% (0.9 % NaCl) and 60.01% (RL). The conclusion of this study that the quality of spermatozoa with 0.9% NaCl and RL diluent decreased during storage at room temperature, the sperm quality until the 30th minute still fulfilled the requirements for AI application, and the best egg fertility was shown in the use of 0.9% NaCl diluent.

Pengaruh NaCl Fisiologis dan Ringer Laktat terhadap Kualitas Spermatozoa pada Suhu Ruang dan Fertilitas Telur Ayam Buras