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Lemongrass oil derived from some species of grass in the family of Poaceae (particularly, Cymbopogoncitratus) posses a highly effective insect repellent potential. In Vietnam, this product is widely commercially available but its quality is not strictly controlled. From a formulator's perspective, lemongrass essential oil is not suitable for direct application on the skin because high concentration of citral, a major chemical constituent of this oil, may cause local irritation. In addition, this compound is volatile, resulting in a short repellent effect. Contributing to solve these problems, a high-performance liquid chromatography with diode array detection was developed for the simultaneous quantification of neral and geranial, two geometric isomers of citral. This method was used to examine the quality of some lemongrass oil samples in order to choose material for the preparation of insect repellent cream. This experimental research demonstrates that the stability of the lemongrass oil cream containing 6% of citral was significantly improved when using βcyclodextrin, a cyclic oligosaccharide capable of protecting substances by capturing them in a blunted-cone shaped structure. The obtained product shows insect repellent effect against the banded sugar ant (Camponotus consobrinus). The effect did not change after a six-month storage in conventional conditions.

tạp chí khoa học đại học quốc gia hà nội: khoa học y dược (vnu journal of science: medical and pharmaceutical sciences)

Validation of a High-Performance Liquid Chromatographic Method with Diod Array Detection for the Quantification of Citral and Formulation of Insect Repellent Cream from Lemongrass Oil