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The diversity of chloroplast DNA (cpDNA) of citrus was revealed by means of a cleaved amplified polymorphic sequence (CAPS) using 97 Citrus, including many local accessions from Japan, China, and Indonesia, four Fortunella, and two Poncirus. All accessions were classified into seven types based on the results of polymorphic bands in all primer/enzyme combinations. The seven types were identified as follows: Type 1 (38 accessions): C. macroptera, C. hystrix, C. aurantifolia, C. medica, C. limon, C. grandis, C. aurantium, C. myrtifolia, C. bergamia, C. rokugatsu, C. sinensis, C. sphaerocarpa, C. nobilis (Kunenbo), C. keraji, C. oto, C. tarogayo, C. suavissima, mandarins of Yunnan and Guangxi, China, miscellaneous of Yunnan, China, C. madurensis, and Fortunella; Type 2 (one accession): C. latipes; Type 3 (two accessions): C. ichangensis and C. junos; Type 4 (six accessions): C. tachibana and C. depressa; Type 5 (18 accessions): C. jambhiri, C. tankan, C. sunki, C. reshni, C. depressa, mandarins of Kagoshima, Japan and Yunnan and Guangxi, China; Type 6 (36 accessions): C. nobilis (King), C. unshiu, C. reticulata, C. genshokan, C. clementina, C. succosa, C. suhuensis, C. tardiferax, C. erythora, C. kinokuni, C. oleocarpa, C. leiocarpa, mandarins of Kagoshima, Japan, Guangxi, Zejiang, Yunnan and Guangdone, China, and mandarins of West Sumatra, Indonesia; Type 7 (Two accessions): Poncirus. The differentiation of cpDNA in citrus was discussed in accordance with the results.日本中国およびインドネシアの在来カンキツを含むカンキツ属97種類キンカン4種類およびカラタチ2種類を供試してcleaved amplified polymorphic sequenceCAPS分析による葉緑体 DNAcpDNAの多型について検討した全てのプライマーと制限酵素の組み合わせで出現したフラグメントのタイプから供試種は7種類に区分できたタイプ138 種類C. macropteraC. hystrixC. aurantifoliaC. medicaC. limonC. grandisC. aurantiumC. myrtifoliaC. bergamiaC. rokugatsuC. sinensisC. sphaerocarpaC. nobilisクネンボC. kerajiC. otoC. tarogayoC. suavissima中国雲南および広西のマンダリン中国雲南の雑種C. madurensisFortunellaタイプ21種類C. latipesタイプ32種類C. ichangensisC. junosタイプ46種類C. tachibanaC. depressaタイプ518種類C. jambhiriC. tankanC. sunkiC. reshniC. depressa鹿児島並びに中国雲南および広西のマンダリンタイプ636種類C. nobilisキングC. unshiuC. reticulataC. genshokanC. clementinaC. succosaC. suhuensisC. tardiferaxC. erythoraC. kinokuniC. oleocarpaC. leiocarpa鹿児島中国広西浙江雲南および広東並びにインドネシア西スマトラのマンダリンタイプ72種類Poncirus. これらの結果に基づいてカンキツにおける葉緑体DNAの分化について考察した

Diversity of Chloroplast DNA in Various Mandarins (Citrus spp.) and Other Citrus Demonstrated by CAPS Analysis