English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Fluorescent banding patterns of pear chromosomes were determined from samples taken from root tips of open-pollinated seedlings of Pyrus pyrifolia (Burm. F) Nakai (Japanese pear), P. calleryana Decne. (Callery pear), P. pyrifolia × P. ussuriensis var. aromatica (a hybrid of Japanese pear and Iwateyamanashi), and P. mikawana Koidz. (Toyotomi Nashi). All accessions used in this study had 2n = 34 chromosomes. Chromomycin A3 (CMA)-positive (+) bands were observed in telomeric positions of four chromosomes in all accessions. 4'-6-diamidino-2-phenylindole (DAPI)-negative bands (−) corresponded with CMA+ bands. Fluorescence in situ hybridization (FISH) was conducted using open-pollinated seedlings of ‘Osa Gold’ (Pyrus pyrifolia) and Toyotomi Nashi (P. mikawana) as materials. Four out of six 18S-5.8S-25S ribosomal RNA gene (rDNA) sites corresponded with CMA+/DAPI− bands. The 5S rDNA sites were detected in centromeric positions of two chromosomes. Two centromeric 5S rDNA and six telomeric 18S-5.8S-25S rDNA sites were located on different chromosomes as determined from the results of multi-color FISH.ニホンナシ‘埼玉8’Pyrus pyrifoliaBurm. F.マメナシ‘愛知マメナシ’P. calleryana Decne.ニホンナシとイワテヤマナシとの交雑種‘岩手7号’P. pyrifolia × P. ussuriensis var. aromaticaおよびトヨトミナシP. mikawana Koidz.の自然交雑実生を用いて染色体の蛍光染色を実施した供試全系統の染色体数は 2n = 34 であったクロモマイシン A3CMA染色を行うと全系統で4本の染色体の端部に CMA+ バンドが確認できた4'-6-ディアミディノ-2-フェニルインドール二塩酸塩 (DAPI)− バンドの位置は CMA+ バンドと一致した続いて蛍光 in situ ハイブリダイゼーションFISHを‘おさゴールド’Pyrus pyrifoliaおよびトヨトミナシ自然交雑実生を用いて行った6ケ所の 18S-5.8S-25S rDNA 遺伝子のうち4ケ所は CMA+/DAPI− バンドの位置と一致した5S rDNA 遺伝子は2本の染色体の動原体近傍に確認できた動原体近傍の2ケ所の 5S rDNA と端部の6ケ所の 18S-5.8S-25S rDNA 遺伝子は各々別の染色体に存在した

Fluorescent Staining and Fluorescence in situ Hybridization of rDNA of Chromosomes in Pear (Pyrus spp.)