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Etiolated taro (Colocasia esculenta Schott) stem cultured under dark were used to study callus formation and plant regeneration. Aseptic plantlets derived from shoot apices of 'Eguimo', Ishikawa-wase' and Tatsugashira' corms were used as explants. 1. Etiolated stems were obtained by culturing them under dark condition on MS medium supplemented with 0.2 mg•Eliter-1 NAA (Table 1); their elongation was not promoted by a supplement of 1.0 mg•Eliter-1 of GAS. 2. The longest stem was obtained from Ishikawa-wase', followed by that of 'Eguimo' and then 'Yatsugashira' (Table 2). 3. Friable callus was obtained from etiolated stem segments on MS medium supplemented with 2 mg•liter-1 2,4-D and 2ip (Table 3), whereas protocorm-like structure developed on a medium containing 0.2 to 2.0 mg•liter-1 NAA plus 2 mg•liter-1 BA or 2ip. 4. Shoots were regenerated from a protocorm-like structure by transferring to MS medium (Table 4) or from callus by subculturing them on MS medium containing 2 mg•liter-1 BA (Table 5). These shoots formed roots after being transferred to a basal MS medium.

Callus Formation and Plant Regeneration from Etiolated Stem of Taro(Colocasia esculenta Schott).