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The induction ofpolyphenol oxidase (PPO) gene mutation through ion beam irradiation ofprotoplasts was attempted for the production ofle飢lceplants with a low browning prope向ras a cut vegetable. It was clarified that the optimum protoplast density was 0.63-0.75 x 10mLー1for shoot regeneration from protoplasts of lettuce‘Round'. Effects of 12C6+ ion beam irradiation on the growth of protoplasts were investigated, and the percentage of colony forrnation decreased as the irradiation dose increased. The dose leading to 50% cel growth as compared with the untreated control was approximately 3.3 Gy. Two out of 869 cali irradiated with 12C6+ ion beams were found to be PPO gene mutations by the TILLllぜGmethod. PPO activities of the re幽 generated plants from the two mutant cali named 2Gy-3-36 and 5Gy・2司 27were preliminarily assayed, and lettuce plants企om 2Gy-3-36 exhibited half of the PPO activity as compar巴dwith the non-irradiated controls. A part of the M2 population企om 2Gy-2-36 exhibited the property ofno band ofheteroduplex cleavage by Cel 1, with markedly low PPO activity as the control.

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Low Polyphenol Oxidase Mutant Induced by &lt;sup&gt;12&lt;/sup&gt;C&lt;sup&gt;6+&lt;/sup&gt; Ion Beam Irradiation to Protoplasts of Lettuce (&lt;i&gt;Lactuca sativa&lt;/i&gt; L.)