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Bacterial wilt of eggplant was suppressed by inoculation with non-pathogenic Ralstonia solanacearum (PC (phenotypic conversion) mutants). We tested simplified evaluation methods to select effective PC mutants for inoculation. Seeds and 1-leaf seedlings were inoculated with PC mutants. After these grew into plants with 3–4 leaves, they were inoculated with the wildtype strain. Seeds inoculated with PC mutants at the 1-leaf stage showed suppression of bacterial wilt. One-leaf seedlings were inoculated with PC mutants and then transplanted to soil inoculated with the wild-type strain as an easier method—seedling inoculation method. These plants also showed suppression of bacterial wilt. We investigated the suppression of bacterial wilt by PC mutants derived from 10 wild-type strains using the seedling inoculation method. Four strains that showed a highly suppressive effect were selected.

Simplified Evaluation Method for Suppressing Bacterial Wilt Disease by Treating Non-pathogenic Ralstonia solanacearum Mutant Selection in Eggplant