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Virus-induced gene silencing (VIGS) systems are widely used to downregulate target host genes in plants. The Cucumber mosaic virus (CMV), which has a broad host range, has been used as a VIGS vector system in research. In this study, a VIGS vector system derived from the pepo strain of CMV (CMV pepo) was tested for its use in functional analysis of a target gene in Petunia. The CMV pepo caused systemic infections without severe viral symptoms in petunia plants. A vector containing a short fragment (57 bp) of the chalcone synthase gene (PhCHS-A) was constructed (PhCHSA-CMVpepo) and petunia plants were mechanically inoculated with it. Corollas were white in plants infected with PhCHSA-CMVpepo, but were purple (original color) in plants infected with wild-type CMV pepo. In white corollas, the mRNA level of PhCHS-A was downregulated compared to that in purple corollas. These results indicate that the CMV pepo vector system is useful for VIGS research in flowers of the genera Petunia.

horticulture journal

Silencing of the &lt;i&gt;Chalcone Synthase&lt;/i&gt; Gene by a Virus Vector Derived from the &lt;i&gt;Cucumber Mosaic Virus&lt;/i&gt; in &lt;i&gt;Petunia&lt;/i&gt;