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In this study, ıt was aimed the investigate the effects on Pirlak ram spermatological parameters added sperm extender which antioxidant properties of Trolox and Taurin. For this aim semen was collected with the aid of artificial vagina twice a week from 10 Pirlak ram. Semen which were normospermic pooled and occurred three group; control, 1 mM Trolox, 50 mM Taurin. Groups were equilibrated about 2 hours at + 4 ° C, frozen in liquid nitrogen vapor (-120 ° C), stored in liquid nitrogen. Pos-thawed spermatological examinations; motility was recorded subjectively, plasma membrane integrity (PMAI), high mitochondrial membrane potential (HMMP) and mitochondirial oxidative damage level (MITOSOX+) were evaluated by flow cytometer. Post-thawed motility ratio was higher in 1 mM Trolox (%50.00±3.89) and 50 mM Taurin groups compared the control (35.00±2.11) group (p<0.05). Taurin 50 mM (%32.42±0.72) was highest PMAI ratio and it was significantly important with control group (P<0.05). HMMP levels were higher in antioxidant groups when compared the control group and also lowest mitochondirial oxidative damage level was seen in the 50 mM Taurin (P<0.05). Finally, when freezing of Pirlak ram semen in the out of breeding season Trolox and Taurin which were added the semen extender was ameliorating effect on post thaw motility and HMMP levels. Behind this Taurin 50 mM gave the best preventive effect on oxidative damage.

Koç Spermasının Dondurulmasında Oksidatif Stres Üzerine Trolox ve Taurinin Etkisi