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Objective: Assuming that obesity and chronic stress are both physiological disturbances that alter the homeostasis in mammalian, the aim of this study is to analyze the effect of both conditions on biochemical and hematological parameters in female Wistar rats. Methods: Animals were fed with rodent standard feed or cafeteria diet and, from the eighth week, grouped in (a) standard diet (Std); (b) Std + stress; (c) cafeteria diet (Cafe) and (d) Cafe + stress. The animals of groups non-stressed control and stressed control were fed with rodent standard feed and those of groups non-stressed obese and stressed obese, besides the standard feed, were offered a cafeteria diet (variety of processed food). The following parameters were evaluated: body mass (weekly), hematological parameters (erythrogram and leukogram), glycemia, lipidogram, relative mass of adrenal gland and visceral and retroperitoneal fat. Results: The cafeteria diet was effective in inducing obesity, while stress induced by restriction caused an increase in the relative adrenal gland mass, a recognized stress parameter. There was no alteration in the biochemical or in the hematological parameters caused by the interaction of obesity and chronic stress. However, obesity was able to induce hyperglycemia and leukocytosis driven by neutrophilia and lymphocytosis. Conclusion: We conclude that the cafeteria diet used in this study is effective in inducing obesity in female Wistar rats, but association with chronic stress did not alter hematological and biochemical parameters.

Cafeteria Diet Associated with the Chronic Stress Does Not Alter Biochemical and Hematological Parameters in Wistar Rats