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Biosensing devices such as assays require both speed and sensitivity as two integral components for its effectiveness. Within this review, developed three-dimensional (3D) microfiber polystyrene (PS) platforms are leveraged to introduce a means for effectively rapid and sensitive assays. Considered broadly, the 3D structure contributes to an increased surface area for antibody immobilization while the natural hydrophobic nature of PS promotes this immobilization for immunoassay diagnostics. More specifically, rapid antigen capture can be realized by combining this platform with vacuum pressure to mitigate diffusion limitations for antibody-antigen interactions. Alternatively, the increased antibody immobilization density can realize the selective capture and release of circulating tumor cells (CTC). Through both low concentration CTC capture and effective release of said cells, patient-specific cancer care can be achieved.

Electrospun Polymeric Microfiber Substrates for Rapid Protein and Cell-based Assays