English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

When vitamin K deficiency occurs, precursor forms of vitamin K dependent coagulation factors which are termed protein induced by vitamin K absence (PIVKAs) appears in the circulating blood. Crossed immunoelectrophoresis has usually been employed to demonstrate the presence of PIVKAs, but the procedure is time consuming for clinical use. In the present test method developed by the authors, the abnormal form of prothrombin (PIVKA-II) in the plasma can readily be determined within a period of 30 min. This test method is a simple and rapid assay for the detection PIVKA-II. The principle of this assay is based on the agglutination of antihuman prothrombin rabbit IgG coated latex (AP-Latex) with absorbed plasma which is treated with barium carbonate. After mixing with 25, 11 of the plasma and 200; 1 of the 0.2% bovine albumin saline, in which 100mg/ml of bairum carbonate was suspended, incubation at 37C or room temperature for 10 min was carried out with shaking every 2 min. After the incubation, 275 pl of the bovine albumin saline solution was added and the mixture was centrifuged at 3000 rpm for 5 min. Then 100 pl of the supernatant placed on a glass slide (2X3cm), one drop of Ap-Latex was added. After the mixture was shaked for 2 min, the latex agglutination was checked in daylight against a flat dark background. The level of PIVKA-II was expressed by the maximum dilution level being agglutinated in the specimen. This assay is expected to be useful as a screening test to differentiate whether or not vitamin K deficiency is present in various clinical cases.

A simple and rapid method for the assay of PIVKA-II and its practical use for the clinical cases