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For the assay of gastric glandular kallikrein of the rat, a synthetic substrate was used, the structural formula of which is as follows; L-Pro-L-Phe-L-Arg-aNE. The hydrolytic activity of sample solution was used as an esterolytic activity. The influence of the blood and peptic fluid mixed into the sample solution could be negated. When low molecular weight kininogen was added to the sample solution from the glandular stomach which exhibited high esterolytic activity, a significant kallidin value was manifested. Such high correlation coefficient as r=0.953 was detected between an esterolytic activity of the sample solution and kallidin value. From this result, the assay of glandular kallikrein came to be possible. The glandular kallikrein activity was demonstrated. During the process of development of the experimental gastric ulcer due water immersion stress, the glandular kallikrein showed an intimate relationship with tissue plasminogen activator. In the group of rats administered orally with 300 mg/kg of cetraxate, the glandular kallikrein and tissue plasminogen activator did not show any obvious alteration irrespective of the progress in stage during the development of the experimental gastric ulcer due to water immersion stress.

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(II) Glandular kallikrein of rat stomach: Behavior of glandular kallikrein and tissue plasminogen activator in experimental gastric ulceration occurrence