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The pathogenesis of thrombosis and embolic disorders has been partially clarified. This is related to patients with deficiency or abnormalities of antithrombin III, protein C (PC) and patients with aquired lupus anticoagulant (LA). However, most of the patients showed no abnormal laboratory findings, even though they had thrombosis or embolic disorders. Recently, several reports have suggested the presense of patients with thrombophilic disposition and poor anticoagulant response to activated protein C (APC). We have measured poor anticoagulant response to APC in healthy subjects. Results obtained herein are as follows: 1)The data of plasma samples were affected by freezing procesures and numbers of platelets. Therefore, freshly availabie platelet poor plasms was recommended. 2)The distribution of APC ration in healthy subjects were 2.0•`4.0.

Studies on the Effects of Platelets and Storage in Freeze on the Determination of Poor Anticoagulant Response to Activated Protein C