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Factor VII has been purified from bovine plasma with an over yield of 60%. The isolation procedure involves barium citrate adsorption and elution, ammonium sulfate fractionation (40-70%), DEAE-Sepharose Fast Flow column chromatography, and affinity column chromatography using anti-factor VII monoclonal antibody. The final product was homogenous when examined by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. Using this rapid purification method, 24.6mg and 20mg of factor VII was obtained from 70 liters and 75.3 liters of bovine plasma, respectively.

An Establishment of Large-scale and Rapid Procedure for the Isolation of Factor VII from Bovine Plasma