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Virgin coconut oil has been made by using papaya leaf extract as a source of the papain enzyme. Papaya leaf extraction with maceration used ethanol 95% as solvent. The crude ethanol extract was purified by means of gradient column chromatography using hexane, diethyl ether and ethanol as solvents. The results showed that using papaya leaf extract could significantly increase the quantity of VCO. Coconut milk cream without treatment (negative control) obtained 3.0042 ± 0.046g of VCO, while treatment with papaya leaf extract gained 6,039 ± 0.049 7,952 ± 0.031g of VCO, an increase of about 97.5% 161%. Based on the medium chain saturated fatty acids (MCFA) and long chain saturated fatty acids (LCFA) in VCO, it indicated that the quality of VCO obtained was not good. VCO in diethyl ether fraction and crude extract etanol produced yellow VCO, indicating chlorophyll was still there. Etanol fraction of VCO provided the best quality with the most of lauric acid content and clear color. The VCO components identified using GCMS analysis obtained several fatty acids such as capric acid, lauric acid, myristic acid, palmitic acid, oleic acid, stearic acid, and stearic epoxy.

PEMBUATAN VIRGIN COCONUT OIL DENGAN PENAMBAHAN ENZIM PAPAIN DARI EKSRAK DAUN PEPAYA (Carica papaya)