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Sucrose is well known as naturally occurring sweeteners. Photoreactive sucrose derivative containing 3(trifluoromethyl)diazirinyl moiety is designed for photoaffinity labeling. As 1’-hydroxyl group of sucrose is well known to be less reactive than other primary alcohols, the optimization of reaction conditions for diazirinyl benzyl bromide derivative at sucrose 1’-postion was examined to elucidate the functional analysis of sweet receptors.

Optimization of sucrose 1’-position modification with 3-(trifluoromethyl)diazirinyl benzylbromide derivatives for photoaffinity labeling