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ISOLATION AND SCREENING OF FRESH WATER MICROALGES AS A SOURCE OF CAROTENOID PIGMENT Microalgae are source of bioactive compounds that have potential as a source of functional food for antioxidants such as carotenoids. The aims of this study were to isolated microalgae from freshwater sources and to screening carotenoids from biomass microalgae. Microalgae were isolated using micropipettes and were cultivated in bold’s basald medium (BBM). Determination of growth rates was performed using a UV-Vis spectrophotometer,dry biomass was determined using gravimetric and total carotenoid screening was carried out using the Lichtenthaller method employing a UV-Vis spectrophotometer. Four species of microalgae were obtained with a green color indicating that all of them are chlorophyta (green algae) microalgae. Screening total carotenoid was carried out on four isolated microalgae species and two microalgae species were obtained from biochemistryy laboratory such as Scenedesmus rubescens and Galdiera sulphuraria. The highest total carotenoid content was microalgae isolated from pond water with belong to chlorophyta genus with carotenoid content 36 ± 0.25 μ/g. These results provide information that the isolated microalgae can be used as potential as a source and production of carotenoids.

ISOLASI DAN SKRINING MIKROALGA AIR TAWAR SEBAGAI SUMBER PIGMEN KAROTENOID