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The effects of water stress on stomatal aperture and epidermal cells of attached sunflower leaves, were observed with a remote-control light microscope system. The relationships between stomatal movements and changes in water potential, turgor potential and osmotic potential of attached leaves were examined. The leaf water deficit was caused by ice water perfusion to roots and the recovery was performed by 20•Ž water perfusion. After the ice water perfusion the stomata immediately opened with severe turgor loss and hollow of epidermalcells, and then it rapidly closed. The severe water deficit (leaf water potential: -0.5 to -1.3 MPa) produced complete closure of stomata but the slight water deficit (-0.5 to -0.8 MPa) caused incomplete closure and immediate recovery after 20•Ž water perfusion. The hollow of epidermal cells indicated a time point of 0 MPa turgor

nougyou kishou/nogyo kisho

Study on changes in stomata and their surrounding cells using a nondestructive light microscope system: Responses to changes in water absorption through roots.