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Effects of infection time by Fusarium graminearum on ear blight, deoxynivalenol and zearalenone production in wheat Although it is generally agreed that most Fusarium head blight infection takes place during the wheat flowering period, very rainy conditions at the end of the plant growth, such as occurred in France in 2007, raise the question of the possibility of late infections on the development of the disease and the production of toxins. Such infections are not taken into account by predictive models. To explore the effect of infection time on disease severity, Fusarium-damaged kernels (FDK), desoxynivalenol (DON) and zearalenone (ZON), an experiment was set up in 2009 on two varieties. The experiment had a split-plot design. Plots were inoculated by spraying Fusarium graminearum conidia at different stage, from heading to ripening. Inoculation time had a significant effect on disease severity, FDK, DON and ZON levels (P<0.0001), as well as interaction between variety and inoculation time. The results of this trial have shown no significant difference between infection at heading, anthesis and post-anthesis until 15 days for disease severity, FDK and mycotoxins levels. Nevertheless, this trial has suggested different behaviour between the two varieties according to infection time and toxin accumulation. The period of high susceptibility to Fusarium graminearum has appeared unstable between varieties, ranging from closed to flowering to a large period from heading to several days after flowering.