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The shell, radula, penis and female reproductive organs of Alzoniella slovenica (Lozek et Brtek, 1964), from Slovakia, are described. The morphology of A. slovenica resembles the literature description of A. hartwigschuetti Reischütz, 1983 from Austria, and is less similar to three Alzoniella species from Italy. Sequences of cytochrome oxidase subunit I (COI) were used for phylogenetic reconstruction. The closest relatives of A. slovenica are Radomaniola and Anagastina, not the Italian Alzoniella finalina Giusti et Bodon, 1984. A. slovenica is not congeneric with A. finalina, in which case morphological characters are misleading.

Phylogenetic relationships of <i>Alzoniella slovenica</i> (Ložek et Brtek, 1964) (Caenogastropoda: Hydrobiidae)