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Different methods were used to detect antibacterial activity of Lactobacillus rhamnosus against some food spoilage microorganisms. This study demonstrates that in comparison with culture supernatant and sonificated cells of L. rhamnosus the strongest antimicrobial activity was observed when live test culture was used. It can be suggested that the best results were seen with two methods: the dual culture overlay assay and the agar slab method. From comparison between methodologies it can be concluded that the agar slab technique gives the most reliable and reproducible results.

annales universitatis mariae curie-skłodowska. sectio c, biologia

Comparison of different methods for detection of antimicrobial activity of probiotic strains of Lactobacillus rhamnosus against some food spoilage microorganisms