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Our previous research showed that the best yield of virus-like particles (VLPs) formed by RNA bacteriophage GA coat protein was obtained by expression in yeast Pichia pastoris, while other used expression systems in Saccharomyces cerevisiae gave much lower amounts of capsids. The main reasons to attempt further studies in Saccharomyces cerevisiae were to improve the yield of GA-based VLPs using constructs with optimised nucleotide triplets in coding sequences, and to exploit the possibilities of the two-promoter Gal1/Gal10 system of expression vector pESC-URA for production of the desired mosaic VLPs and for packaging of mRNAs into VLPs in vivo 9 9 Mūsu iepriekšējie pētījumi parādījuši, ka lielākais RNS bakteriofāga GA apvalka proteīna veidoto vīrusiem līdzīgo daļiņu (VLD) ieguvums ir ekspresējot tās raugos Pichia pastoris; savukārt, izmantojot Saccharomyces cerevisiae ekspresijas sistēmu, kapsīdu ieguvums ir daudz mazāks. Divi galvenie mērķi turpmākajām studijām raugos Saccharomyces cerevisiae bija paaugstināt GA apvalka proteīna veidoto VLD ieguvumu, izmantojot konstrukcijas ar optimizētu nukleotīdu tripletu sekvencēm, un izpētīt iespējas, ko varētu sniegt divu promoteru Gall/GallO saturošs ekspresijas vektors pESC-URA vēlamo mozaīkveida VLD iegūšanai un mRNS iepakošanai VLD in vivo

proceedings of the latvian academy of sciences. section b, natural sciences/latvijas zinātņu akadēmijas vēstis. a daļa, humanitārās un sociālās zinātnes

Expression of GA Coat Protein-Derived Mosaic Virus-Like Particles in Saccharomyces cerevisiae and Packaging in vivo of mRNAs into Particles