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Summary Background: Intestinal obstruction results in distortion of balance of antiinflammatory cytokines and release of oxidants, and also leads to bacterial translocation, sepsis and multiple organ failure. Asymmetric dimethylarginine is related to multiple organ failure as a new prognostic marker. Erythropoietin reduces the inflammatory response by decreasing the levels of proinflammatory cytokines and cytokine-induced apoptosis. In this study, we aimed to investigate the effectiveness of erythropoietin in reducing the severity of bacterial translocation and inflammatory response after intestinal obstruction and the relation between asymmetric dimethylarginine and inflammatory markers. Methods: Forty Wistar albino rats (200-250 g) were divided into 4 groups as follows: Group 1 (Sham), only ileocaecal junction dissection; Group 2 (Erythropoietin), ileocaecal junction dissection and 3000 lU/kg erythropoietin subcutaneously; Group 3 (Intestinal Obstruction), complete ileal ligation; Group 4 (Intestinal Obstruction + Erythropoietin), complete ileal ligation and 3000 IU/kg erythropoietin subcutaneously. After 24 hours, the rats were sacrificed by taking blood from the heart for biochemical analyses. Peritoneal swab culture, liver, mesenteric lymph nodes, spleen and ileum were collected for microbiological and histopathological examinations. Results: Erythropoietin reduced the secretion of inflammatory cytokines, oxidative damage and bacterial translocation, prevented the formation of inflammatory changes in the intestine, liver, spleen and mesenteric lymph nodes,and also significantly prevented the formation of intestinal damage after intestinal obstruction (p<0.05). Conclusions: Asymmetric dimethylarginine levels did not differ between the groups. Erythropoietin may be useful to preserve from intestinal injury and related sepsis in patients with intestinal obstruction. Asymmetric dimethylarginine is not a suitable prognostic marker. Kratak sadržaj Uvod Kao posledica intestinalne opstrukcije javlja se poremećaj ravnoteže antiinflamatornih citokina i oslobađanje oksida nata, a dolazi i do bakterijske translokacije, sepse i višestrukog otkazivanja organa. Asimetrični dimetilarginin dovodi se u vezu sa višestrukim otkazivanjem organa kao novi prognostički marker. Eritropoetin ublažava inflamatorni odgovor smanjivanjem nivoa proinflamatornih citokina i apoptoze izazvane citokinima. U ovoj studiji cilj je bio da istražimo efikasnost eritropoetina u pogledu smanjenja obima bakterijske translokacije i inflamatornog odgovora posle intestinalne opstrukcije, kao i odnos između asimetričnog dimetilarginina i inflamatornih markera. Metode: Četrdeset albino pacova soja Wistar (200-250 g) podeljeno je u sledeće četiri grupe: Grupa 1, samo disekcija ileocekalnog spoja; Grupa 2 (Eritropoetin), disekcija ileocekalnog spoja i 3000 IU/kg eritropoetina supkutano; Grupa 3 (Intestinalna opstrukcija), kompletna ilealna ligacija; Grupa 4 (Intestinalna opstrukcija + eritropoetin), kompletna ilealna ligacija i 3000 IU/kg eritropoetina supkutano. Posle 24 časa, pacovi su žrtvovani uzimanjem krvi iz srca za biohemijske analize. Kulture peritonealnog brisa, jetre, mezenteričnih limfnih čvorova, slezine i ileuma uzete su za mikrobiološka i histopatološka ispitivanja. Rezultati: Eritropoetin je smanjio lučenje inflamatornih citokina, oksidativno oštećenje i bakterijsku translokaciju, sprečio nastanak inflamatornih promena u crevima, jetri, slezini i mezenteričnim limfnim čvorovima i u značajnoj meri takođe sprečio nastanak intestinalnih oštećenja posle intestinalne opstrukcije (p<0,05). Zaključak: Nivoi asimetričnog dimetilarginina nisu se razlikovali između grupa. Eritropoetin može biti koristan za sprečavanje intestinalnih oštećenja i sepse kod pacijenata sa intestinalnom opstrukcijom, dok asimetrični dimetilarginin nije odgovarajući prognostički marker.

journal of medical biochemistry

The Effects of Erythropoietin on Bacterial Translocation and Inflammatory Response in an Experimental Intestinal Obstruction Model in Rats / Uticaj Eritropoetina Na Bakterijsku Translokaciju I Inflamatorni Odgovor U Eksperimentalnom Modelu Intestinalne Opstrukcije Kod Pacova