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Mitogen-activated protein kinase (MAPK) cascades participate in various processes, including plant growth and development as well as biotic and abiotic stress responses. MAPK kinases (MKKs), which link MPKs and MPKK kinases, are involved in MAPK cascades by mediating various plant stress responses. However, only a few MKKs from Brassica campestris (rape) have been functionally characterized. This study delivers the results from isolation and characterization of a novel gene, MKK2, from rape. Bioinformatics analysis revealed that the cDNA length of MKK2 is 1,344 bp with an open reading frame of 1,068 bp, which encodes a polypeptide containing 355 amino acids. The obtained MKK2 exhibited a predicted molecular mass of 39.3 kDa and an isoelectric point of 6.8. Quantitative real-time polymerase chain reaction analysis revealed that MKK2 expression can be induced by cold and salt. Western blot analysis revealed that MKK2 protein expression can be induced by cold, salt, and UV-B radiation. The MKK2 protein was localized in the nucleus. These results suggest that MKK2 is important for the regulation of cold- and salt-stress responses in plants.

Identification of a novel mitogen-activated protein kinase kinase gene (MKK2) in the oilseed rape Brassica campestris