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Heat shock protein 70 (HSP70) is an important member of the heat shock protein family. It plays a key role in the process of protecting cells by facilitating the folding of nascent peptides as well as the cellular stress response. We isolated and sequenced a full-length HSP70 cDNA from planarian Dugesia japonica (designated Djhsp70) using rapid amplification of cDNA ends (RACE). Djhsp70 cDNA is highly homologous to other reported hsp70 family genes, and the deduced amino acid sequence shares several eukaryotic HSP70 family motifs. The length of the Djhsp70 ORF from planarian genomic DNA and cDNA was identical, which indicated the absence of introns in the Djhsp70 gene. Semi-quantitative RT-PCR was employed to detect the expression levels of Djhsp70 in response to stressors. Our results indicated that Djhsp70 was an inducible gene, expressed in response to temperature changes, amputation and starvation. Interestingly, the phylogenetic analysis of DjHSP70 supports the idea that planarians belong to a new phylogenetic position — Lophotrochozoans. This is the first molecular analysis of a heat shock protein gene in planarians.

Cloning and expression analysis of hsp70 gene from freshwater planarian Dugesia japonica