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A method for extracting coenzyme Q10 (CoQ10) from Artemia was developed. 1 g of fresh Artemia was incubated with 75 % acetic acid at (30 ± 2)°C for 24 h, followed by three consecutive extractions with a mixture of 5 mL of hexane and 5 mL of ethanol, then analysis by a validated high-performance liquid chromatography with a diode-array detector. The calibration curve for CoQ10 was linear in a range of 1–50 μg mL−1. The limits of detection and quantification were 0.3 μg mL−1 and 1.1 μg mL−1, respectively. Mean recoveries were 94–100 % with a high precision of below 10 %. The method developed was found to be simple, efficient and the time required for releasing CoQ10 from Artemia was short. The method provides not only low energy consumption but is also practical for industrial applications.

Development of an effective extraction process for coenzyme Q10 from Artemia