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Cereal alkylresorcinols (ARs) are a group of phenolic lipids mainly found in the outer parts of grains of rye and wheat. They have been suggested for use as selective biomarkers for intake of whole grain and bran products of these cereals. Consumption of whole grains and whole grain products has been associated with reduced risk of developing chronic diseases, such as cardiovascular disease, diabetes type 2, obesity and some types of cancer. In this article a sensitive and rapid method of High Performance Liquid Chromatography with UV detection for quantitative determination of ARs in the cereals grown in Latvia is described. Instrument detection limits (IDL) were determined for C17:0, C19:0 and C21:0 homologues (coefficient of variation < 3%). According to the results of these studies, ARs were found in rye (87.1-112.0 mg/100 g), wheat (24.0-40.2 mg/100 g), triticale (32.1-74.4 mg/100 g), and in small amounts in barley (2.2-3.7 mg/100 g), but not in oats. Abstrakt Graudaugu alkilrezorcīni (AR) ir savienojumu grupa, kur viena molekulas daļa ir fenola atlikums, bet otra ir lipīdu atlikums. AR lielā daudzumā atrodas grauda ārējos slāņos (graudapvalkos, aleirona slānī) galvenokārt rudzu un kviešu graudos, kā arī nelielos daudzumos miežos. Rafinētos produktos AR ir niecīgos daudzumos vai vispār nav. Tieši tāpēc AR tiek piedāvāts lietot par selektīvu pārtikas biomarķieri, lai patērētājiem būtu vieglāk saprast, kurš produkts ir pilngraudu vai satur pilngraudus. Pilngraudu produktu patērēšana ir saistīta ar samazinātu risku saslimt ar dažādām hroniskām slimībām: aptaukošanās, 2. tipa diabēts, sirds slimības un vēzis. Šajā publikācijā tiek prezentēta ātra un jutīga augsti efektīvu šķidrumu hromatogrāfija ar ultravioletu detektoru - metode alkilrezorcīnu noteikšanai Latvijā audzētos graudaugos. Instrumenta detektēšanas robeža tika noteikta C17:0, C 19:0 un C21:0 homologiem (variācijas koeficients < 3%). AR saturs rudzos bija robežās no 87,1 līdz 112,0 mg/lOOg, kviešos no 24,0 līdz 40,2 mg/lOOg, tritikālē no 32,1 līdz 74.4 mg/lOOg, nedaudz miežos no 2,2 līdz 3,7 mg/lOOg, bet auzās netika atrasts.

proceedings of the latvian academy of sciences. section b, natural sciences/latvijas zinātņu akadēmijas vēstis. a daļa, humanitārās un sociālās zinātnes

Content of Alkylresorcinols in Cereals Grown in Latvia Determined by High Performance Liquid Chromatography with UV