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Abstract Protein molecules present more complex analytical challenges than conventional low molecular weight pharmaceutical compounds, and thus need powerful analytical approaches for the entire pharmaceutical development and quality control process. We used high-performance liquid chromatography to investigate the reversed-phase chromatographic behavior of eleven proteins (albumin, carbonic anhydrase, cytohrome c, L-glutamic dehydrogenase, enolase, α-lactoglobulin, Lysozyme, myoglobin and ribonuclease. By using a water/organic solvent/trifluoroacetic acid system the influence of experimental parameters was examined, and chromatographic results from two C4- chain-length supports were found to be comparable. The model enables prediction of initial conditions from two experimental data points for different types of reversed-phase columns with water-acetonitrile-TFA, water-methanol-TFA, and water-2-propanol-TFA mobile phases Kopsavilkums Olbaltumvielu molekulas ir pēc uzbūves sarežģītākas nekā mazmolekulārie savienojumi, tāpēc ir nepieciešams pētīt risinājumus olbaltumvielu analizēšanai.Tika izmantota augsti efektīvā šķidruma hromatogrāfija, lai izpētītu vienpadsmit olbaltumvielu (albumīna, karboanhidrāzes, citohroma c, Lglutamāta dehidrogenāzes, enolāzes, α-laktoglobulīna, β-laktoglobulīna, lizocīma, mioglobīna un ribonukleāzes) uzvedību apgrieztās fāzes hromatogrāfijas apstākļos uz divām dažādām C4 kolonnām ar ūdens/organiskais šķīdinātāja/trifluoretiķskābes kustīgajām fāzēm. Mūsu pārbaudītais teorētiskais modelis ļauj prognozēt izdalīšanās laikus, izmantojot divus eksperimentālo datu punktus uz dažāda veida apgrieztās fāzes kolonnām ar ūdens-acetonitrila-TFA, metanola-ūdens-TFA un ūdens-2- propanola-TFA kustīgām fāzēm

latvian journal of chemistry/latvijas ķīmijas žurnāls

The Behavior of Proteins on Reversed-Phase Supports During High-Performance Liquid Chromatography and Prediction of Protein Retention Time on Ymc Columns