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Leaf area LA, is a plant biometric index important to agroforestry and cropproduction. Previous works have demonstrated the conservativeness of theinverse of the product of the fresh leaf density and thickness, the so-calledHughes constant, K. We use this fact to develop LAMM, an absolute method of LAmeasurement, i.e. no regression fits or prior calibrations with planimeters.Nor does it require drying the leaves. The concept involves the in situdetermination of K using geometrical shapes and their weights obtained from asubset of fresh leaves of the set whose areas are desired. Subsequently theLAs, at any desired stratification level, are derived by utilizing K and thepreviously measured masses of the fresh leaves. The concept was first tested inthe simulated ideal case of complete planarity and uniform thickness by usingplastic film covered card-paper sheets. Next the species-specificconservativeness of K over individual leaf zones and different leaf types fromleaves of plants from two species, Mandevilla splendens and Spathiphyllumwallisii, was quantitatively validated. Using the global average K values, theLA of these and additional plants, were obtained. LAMM was found to be a rapid,simple, economic technique with accuracies, as measured for the geometricalshapes, that were comparable to those obtained by the planimetric method thatutilizes digital image analysis, DIA. For the leaves themselves, there were nostatistically significant differences between the LAs measured by LAMM and bythe DIA and the linear correlation between the two methods was excellent.

Foliar area measurement by a new technique that utilizes the conservative nature of fresh leaf surface density