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Summary Introduction: Bauhinia variegata  is used in traditional medicine in Pakistan. Objective:  The present study was designated to analyze phytochemical screening and cytotoxicity assay of crude aqueous and methanol extracts of flowering buds of  Bauhinia variegata . Methods:  Qualitative and quantitative phytochemical screening of both extracts were performed by using standard protocols while brine shrimp lethality assay was conducted for cytotoxicity analysis of both extracts. Results:  The highest levels of phenolics, flavonoids and terpenoids were observed in methanol extract with respective standard drugs. The aminoacid content was highest followed by phenol and flavonoids in crude aqueous extract of the raw materials. In cytotoxic studies (brine shrimp lethality assay) the use of methanol extract was reported as potent with LD 50  of 241.778  µ g/ml as compared to aqueous extract with LD 50  of 489.7061  µ g/ml. Conclusion:  This study reveals that the presence of bioactive compounds in both extracts are responsible for the treatment of various ailments and being utilized by the traditional folks as a food as well. And showed that methanol extract possess cytotoxic activity and thus needs further scientific intervention for its value addition.

Phytochemical analysis and cytotoxicity evaluation of flowering buds of Bauhinia variegata L.