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This study conducted to determine relationship among and within Iranian  Helichrysum  species (Asteraceae). In this study based on ISSR markers, the highest percentage of ISSR loci polymorphism (54.7%) occurred in  H .  armenium . The highest gene diversity over loci (1.224), Shannon’s Information Index (0.224%) and Expected Heterozygosity (0.142%) occurred in  H .  armenium  (0.18) and the lowest of these parameters (0%) were observed in  H .  araxinum ,  H .  graveolens ,  H .  persicum  and  H .  psychrophilum . The highest genetic similarity occurred between  H .  armenium  and  H .  rubicundum  (0.989), while the lowest was between  H .  polyphyllum  and  H .  graveolens (0.213) . The analysis of molecular variance (AMOVA), showed significant genetic variation among (24%) and within (76%) species. In morphological analysis traits such as indumentum, resting bud, achene length, achenial papillae, dimension of receptacle and form and apex of phyllaries were main diagnostic features. Results obtained from the morphological cluster were greatly consistent with the molecular data, to elucidating taxonomic relationships, as well as both attributed the higher diversity in  H .  armenium  and  H .  rubicundum  in comparison with other species and also indicated that  H. persicum  is a member of  H .  oocephalum  species. Totally we confirmed the presence of 18 species in Iran.

Assessment of relationships among and within Helichrysum Mill. (Asteraceae) species by using ISSR markers and morphological traits