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This paper describes a new bacterial strain which is able to efficiently oxidize arsenite (As(III)) to arsenate (As(V)) in diluted ferrous ion-free 9k medium. The bacteria were isolated from a mine drainage containing arsenic using an arsenite (about 200 mg/l)-containing 9 k medium agar plate. The Bacteria were gram-negative and rod-shaped (0.8 ∼1.4 μm). The oxidation rate of arsenite depended on the cell concentration. Arsenite was oxidized in the pH range from pH4.2 to pH7.5, but the bacteria did not oxidize arsenite below pH3.0. The bacteria derived the energy for the growth from arsenite oxidation and the growth was enhanced with yeast extract.

Oxidation of Arsenite by Bacteria Isolated from Mine Drainage.