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Diosgenin (a steroidal saponin of fenugreek) has lon g been used as a raw substance for the manufacturing of steroid drugs. In order to evaluate the nutritio nal effect of different nitrogen (0, 50, 100, 150 a nd 200 kg ha ) and copper (0, 10, 20 and 30 kg ha ) doses on the diosgenin content, growth and yield o f fenugreek (var. Ardestan), an experiment was carried out in a factori al arrangement based on complete randomized block design. The diosgenin content in the plant was monit ored by a high performance liquid chromatography. Lower doses of copper increased yield, yield compone nt and diosgenin content, but its higher level (30 kg ha) had negative effects on the plant yield. The simu ltaneous application of nitrogen and copper ameliorate the diosgenin production, yield and yiel d component. The result obtained from correlation a nd stepwise regression analysis showed that traits such as leaf area index, pods number per plant, and dry weight have significantly direct relation to diosgen in production levels in plants, while dry weight has the highest effect on diosgenin production compared to other traits. According the results, we also can conc lude that nitrogen application can decrease copper toxic i y in fenugreek in the contaminated soils.

THE EFFECT OF SIMULTANEOUS APPLICATION OF NITROGEN AND COPPER ON YIELD AND STEROIDAL SAPOGENIN PRODUCTION IN TRIGONELLA FOENUM GRAECUM L.