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Senna alata was one of the herbs plants that can be used to treat the ringworm in traditional method. In this study, the senna alata was scientifically tested to prove the traditional medicinal used. Six forms of extract were extracted by using soxhlet apparatus and continued with the appropriate methods that can extract the chemical compound of anthraquinone. The six forms of extraction included anthraquinone aglycone, anthraquinone glycoside, anthraquinone aglycone from glycoside, anthraquinone from crude absolute ethanol, anthraqunone from crude n-hexane and anthraquinone from crude ethanol 70%. The extraction of six forms of anthraquinone extract as antifungal was observed by tested with two types of fungi; Tricophyton rubrum and Microsporum gypseum. The extraction from crude absolute ethanol and crude n-hexane to inhibit these fungi were compared to determine which type of solvent was the best to use for inhibition. This test was implemented by using well-diffusion and broth microdilution method to determine the minimum inhibitory concentration (MIC). The present of six forms of anthraquinone was examined by using Thin-Layer Chromatography (TLC).

EXTRACTION OF CRUDE ESSENTIAL FROM SENNA ALATA (POKOK GELENGGANG)