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New and efficient laccases are needed for degrading lignin in various applications. Therefore, present study aimed at the screening and identification of effective laccase producin fungal strain. A total of 30 fungal strains were isol ed from air, soil and decaying wood of Mangifera indica and screened for their ability to produce laccase on solid medium having tannic acid, guaiacol and syringaldazine as indicator compounds. For quantitative enzyme production, laccase producing strains were grown on fermentation medium having sawdust as a solid substrate. The strain which gave maximum enzyme activity was identified using conventional and molecular methods. Wet and dry lab experiments were performed for determination of better substrate for enzyme using 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid), guaiacol and syringaldazine. Our results showed that out of 30 only 15 strains exhibited laccase producing capability and the strain MFw3 showed highest enzyme activity of 297±1 U/ml. Phylogenetic tree analysis sh ed the novelty of Aspergillus flavus MFw3 (Ge Bank accession number: MF167358). Molecular docking between enzyme and substrate resulted that ABTS exhibited better affinity for laccase with minimum dock score (12.5 Å) as compared to guaiacol (14.7 Å) and syringaldazine (15.5 Å).

INVESTIGATION OF NOVEL laccase PRODUCING FUNGAL SPECIES AND ITS SUBSTRATE SPECIFICITY BY WET AND DRY LAB