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Biosynthesis of Iron nanoparticles from Mentha pulegium L by simple method by using waterextract of Mentha pulegium L. The iron nanoparticles characterized by UV-visiblespectroscopy, (FTIR) Fourier transform infrared spectroscopy, XRD and Scanning electronmicroscopy (SEM). The biological activity evaluation by anti-inflammatory and anti oxidantusing evaluated in vitro 2,2-diphenyl-1- picrylhydrazyl (DPPH) assay, ABTS radicalscavenging assay assay Iron chelating activity assay . The results also revealed that the waterextract of Mentha pulegium L and its iron nanoparticles represented the most potent in vitroantioxidant effect. Iron nanoparticles inhibited the growth of bacterial and fungal, theseresults suggested that iron nano particle could be an interesting to control pathogenic microorganisms.

Studying antioxidant, anti-inflammatory activities and antimicrobial of Iron nanoparticles biosynthesized from water extract of Mentha pulegium L