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INVESTIGATION OF EXTRACELLULAR PROTEASE ACTIVITY OF TWO DIFFERENT EXTREMELY HALOPHILIC ARCHAEA ISOLATED FROM RAW HIDE ABSTRACT. In this study, it was aimed to inves% gate extracellular protease ac% vity of two diff erent extremely halophilic archaea and to iden% fy them by using phenotypic and molecular methods. The haloarchaea were isolated from salted raw hide. The isolates were screened for produc% on of protease enzyme and two isolates which have extracellular protease ac% vity were selected. To iden% fy the halophilic archaea, Gram staining procedure, an% bio% c sensi% vity tests and molecular methods were used. According to the 16S r-RNA molecular iden% fi ca% on results, isolate 1 iden% fi ed as Halococcus morrhuae JCM 8876 with % 99 sequence similari% es and isolate 2 iden% fi ed as Natrinema pallidum 153 with % 99 sequence similari% es. Azocasein was used as a substrate for measuring their proteases ac% vi% es daily and their op% mum ac% vi% es were determined. H. morrhuae and N. pallidum showed daily maximum proteases ac% vity on 6 and 4 days, respec% vely. According to the results of the study, it was found that proteases ac% vity was related with diff erent reac% on condi% ons. These condi% ons were detected as diff erent pH (4-11), temperature (4-70°C) and NaCl concentra% on (1-5 M) values. H. morrhuae showed the maximum ac% vity at pH 9.5, 2M NaCl concentra% on and 50°C temperature and N. pallidum displayed the op% mum ac% vity at pH 9.0, 2M NaCl concentra% on and 40°C temperature.

Investigation of extracellular protease activity of two different extremely halophilic archaea isolated from raw hide