English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

The present study was aimed to test the efficacy of Abelmoschus moschatus trypsin inhibitor (AMTI-II) against the isolated mid gut protease of Helicoverpa armigera. A potent trypsin inhibitor, AMTI-II, has been purif ied and characterized from the seeds of Abelmoschus moschatus following c onventional methods of protein purification and its molecular weight was found to be 21.2 kDa by SDS-PAGE. The inhibitor was quite stable up to 80°C for 10 min and was not affected at alkaline as well as acidic conditions tested. The m id gut protease was found to be homogeneous with a molecular weight of about 30.4 kDa. The isolated protease was alkali st able and its pH optimum for activity was about pH 1 1. The isolated protease was found to be susceptible to inhibition by AMTI-II with an IC 50 value of 2.2 μg. The mode of action of AMTI-II on the mid gut protease was found to be non-competi tive. Results of kinetic studies revealed a low Ki value of 8 x 10 M against the protease. On molar and quantitative bas is, AMTI-II appears to be the most powerful inhibit or than SBTI. AMTIII exerted a strong inhibition on the isolated mid gut protease of Helicoverpa armigera, a property th a helps in exploring its use in the generation of transgenic for combating t he insect pest.

Insecticidal Potential of Abelmoschus moschatus Trypsin Inhibitor (AMTI-II) Against Mid Gut Protease of Helicoverpa armigera