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The aim of this study was to determine The Minimum Inhibitory Concentration (MIC) and the exposure time of the combination of quaternary ammonium compound with ethylenediaminetetraacetic acid (EDTA) towards Methicillin-resistant Staphylococcus aureus so that the combination can be applied as a disinfectant against MRSA.The tested bacteria MRSA was isolated from the RSGM of the Faculty of Dentistry Padjadjaran University. This laboratory experimental study was conducted based on a serial dilution method. The combination of quaternary ammonium compound with EDTA was diluted in 1/1000, 1/2000, 1/4000, 1/8000, 1/16000, 1/32000, 1/64000, and 1/128000 concentration.The result showed that the combination of quaternary ammonium compound with EDTA inhibited the MRSA on 1/1000, 1/2000 and 1/4000 concentrations. The effective exposure time to reduce the number of MRSA colonies are 2.5 and 3 minutes with 1/1000 concentration from the combination. In conclusion that the MIC of the combination of quaternary ammonium compound with EDTA is in 1/4000 concentration and the effective exposure time is more than 3 minutes.

padjajaran journal of dentistry/padjadjaran journal of dentistry

The minimum inhibitory concentration and exposure time of the combination of quaternary ammonium compound with ethylendiamintetraacetic acid (EDTA) against methicillin-resistant Staphylococcus Aureus