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Background and Objectives: L. minor has been used as animal feed, which contains high nutrition, i.e. Crude protein (22,4%), amino acids lysin (6,9%), methionin (1,4%), and hystidin (2,7%), but high crude fiber (10,16%) dan lignin (17,98%). Provision L. minor in poultry is limited to 5%. Therefore, the objective of the study is to get the best quality of L. minor nutrient through fermentation process by using Trichoderma harzianum (Th) and Saccharomyces cerevisiae (Sc). Materials and Methods: L. minor fermentation is divided into two stages: 1.Th (3 x 10 spores /100 grams substrate) with added ZnCO3 (186 ppm) and dl-methionine (286 ppm), II. Sc (3 x 10 spores /100 grams of a substrate). Completed randomized design (CRD) was used in experimental design with 20 experimental units. The treatments consist of P1 (Fermentation using Th for 1 day continued with Sc for 9 days), P2 (Th for 3 days continued with Sc for 7 days), P3 (Th for 5 days continued with Sc for 5 days), P4 (Th for 7 days continued with Sc for 3 days), P5 (Th for 9 days continued with Sc for 1 day) with four replications. Analysis of variance was conducted to know the treatment effect. It was then followed by the Duncan test to know the difference between treatments. Results: Fermentation using Th and Sc has a significant Setiyatwan / Jurnal Ilmu Ternak Desember 2018, 18(2):115-120 116 effect (P<0.05) on lignin, selulos and non nitrogen free extract. The best duration of fermentation is Th for 3 days and Sc for 7 days (P2) that increasing non nitrogen free extract (34,42%), and decreasing lignin (1,25%) and selulosa (10,62%). Conclusion: L. minor fermentation with the combination of Th for 3 days and Sc for 7 days with added dl-methionine and Zn has yielded the highest non nitrogen free extract, and the lowest lignin and selulosa contents.

Kandungan Lignin, Selulosa dan Bahan Ekstrak Tanpa Nitrogen L.Minor Hasil Fermentasi Menggunakan Trichoderma harzianum dan Saccharomyces cerevisiae