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Separation techniques are very necessary in isolating compounds that have bioactivity to obtain pure compounds in development research in the field of organic chemistry of biological natural materials. Compounds isolated from plant tissue are naturally mixed with other compounds, therefore the appropriate separation and purification techniques need to be chosen. High Performance Liquid Chromatography (HPLC) Preparative is one of the equipment in the laboratory that is used to separate and purify compound mixtures with high separability, fast (high throughput). The purpose of this study was to determine the optimum conditions for the separation of flavonoids from the polar fraction of Erythrina poeppigiana using the preparative HPLC system. This research was carried out in the following stages analyzing KCKT analytical polar fraction of E. peppigiana, determining the optimum conditions of preparative HPLC using C18 columns including the concentration of the injected sample, mobile phase selection, and flow rate at 254 nm and 365 nm detection wavelengths. The results showed that the optimum separation conditions of the polar E. poeppigiana fraction were at a sample concentration of 3000 ppm and the mobile phase of a mixture of methanol and water (7:3 v/v) with a velocity flow rate of 5.28 mL / min. The analytical HPLC produced shows one peak and one spot. The optimum conditions produced can be used by researchers for separating the compounds in the polar fraction of E. pepigiana in the laboratory.

jurnal chimica et natura acta/jurnal chemica et natura acta

Optimasi Kondisi Pemisahan Senyawa Flavonoid dari Fraksi Polar Erythrina poeppigiana Menggunakan Alat Kromatografi Cair Kinerja Tinggi (KCKT) Preparatif