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Callus induction of young leaves explant of new clone kamajaya of Durio zibethinus Murr. using the combination of 2,4-D and kinetin in vitro technique Vegetative propagation of new Durian clone Kamajaya can be done with plant tissue culture as a modern technique for germplasm conservation. This study was conducted to determine the combination effects of 2,4-D and Kinetin for callus formation on Kamajaya durian leaves. The research was carried out at the Tissue Culture Laboratory, Faculty of Agriculture, Universitas Padjadjaran, Jatinangor. The experiment design used simple completely randomized design (CRD) factorial with two factors observed and descriptive analysis method. The first factor of plant growth regulator 2,4-D consisted of five different concentrations namely 1, 2, 3, 4, and 5 ppm. The second factor consisted of 3 different concentrations of Kinetin namely 0, 0,5 and 1 ppm. The result showed that 2 ppm 2,4-D, 3 ppm 2,4-D, 4 ppm 2,4-D, 2 ppm 2,4-D + 0,5 ppm kinetin produced rolled up explant of curved young durian leaves and swollen leaf bone. Meanwhile, the treatment of 5 ppm 2,4-D, 2 ppm 2,4-D + 1 ppm kinetin, 5 ppm 2,4-D + 1 ppm kinetin produced rolled up explant of young durian leaves and formed the callus.

jurnal agrikultura/jurnal agribisnis dan agrowisata : the journal of agribusiness and agritourism/jurnal agribisnis dan agrowisata

Induksi Kalus pada Eksplan Daun Muda Tanaman Durian (Durio zibethinus Murr.) Klon Baru Kamajaya dengan Kombinasi Zat Pengatur Tumbuh 2,4-D dan Kinetin Secara In Vitro