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Papaver rhoeas L. (Papaveraceae) is an annual grass native to many regions of the world, which is known to possess several pharmacological activities. It is also known that represent a rich source of anthocyanins. In this study, the content of the anthocyanin was determined in the examined various extracts of fresh petals by HPLC analysis. The most represented anthocyanins in the extracts of Papaver rhoeas L. are delphinidin-3-O-glucoside, cyanidin-3-O-glucoside, cyanidin-3-O-rutinoside, peonidin-3-O-glucoside, petunidin-3-O-glucoside, petunidin-3-acetylglucoside, and delphinidin-3-p-coumaroylglucoside. Acyl derivatives of anthocyanins in the water extract have not been identified. By comparing the contents of individual anthocyanins, the glycosides (polar) are better extracted with 50% alcohol solution, and their acyl derivatives are better extracted with pure alcohol. The water solution is the least suitable for anthocyanin extraction.

HPLC analysis of extracts of fresh petals of Papaver Rhoeas l.