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The synthesis of new 2-(2-(3-formyl-10H-phenothiazin7yl)vinyl)-3H-indolium iodide and comparison of the UV–visible absorption/ emission spectral changes for several indolium cyanine dyes upon variation of organic solvents and aqueous solutions with pH in the range between 5–8 pH units were described.

Optical properties modulation of cyanine dyes in organic solvents and in the critical intracellular pH window