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The purpose of this study was the development and validation of an LC-MS/MS method, for the concomitant and rapid determination amoxicillin and clavulanic acid from human plasma. The sample workup involved a simple protein precipitation procedure. A core/shell type analytical column (50×2,1 mm, 2.6 Å) was used with PFP stationary phase. A mobile phase with high aqueous composition provided satisfactory separation with good accuracy and precision (stable ionization). The mass spectrometer was operated in positive electrospray ionization mode for both analytes and internal standard. The following parameters were evaluated for validation purpose: Selectivity, sensitivity, matrix effect, anticoagulant effect, linearity, precision and accuracy, recovery, analyte/IS stability in solvent/matrix and carryover. The validated calibration range was 190- ng/ml for amoxicillin, and 147-4908 ng/ml for clavulanic acid. The correlation coefficient R2 was at least 0.99 for both analytes. The validated method has been successfully used for the evaluation bioequivalence of generic amoxicillin/potassium clavulanate formulations.

Validated LC-MS/MS Method for the Concomitant Determination of Amoxicillin and Clavulanic Acid from Human Plasma