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Vozdejstvie gipoksii na kletki trofoblasta v processe formirovaniya placenty cheloveka mozhet privodit' k razvitiyu ryada patologij beremennosti, takih kak preeklampsiya i zaderzhka rosta ploda. Patogenez dannyh sostoyanij ne do konca raskryt i mozhet byt' svyazan s izmeneniem ekspressii v trofoblaste ryada genov i regulyatornyh molekul, vklyuchaya mikroRNK. Cel'yu dannogo issledovaniya bylo izuchit' profili mikroRNK i ekspressii sootvetstvuyushchih genov-mishenej v modeli gipoksii trofoblasta. Modelirovanie trofoblasta provodili s ispol'zovaniem kletochnoj linii horiokarcinomy cheloveka BeWo b30. V kachestve induktorov gipoksicheskogo otveta ispol'zovali hlorid kobal'ta (CoCl2) i proizvodnoe oksihinolina. Analizirovali uroven' mRNK i mikroRNK s pomoshch'yu sekvenirovaniya sleduyushchego pokoleniya (NGS) s podtverzhdeniem ekspressii otdel'nyh genov PCR. Analizirovali vtorichnuyu strukturu mRNK gena-misheni znachimo izmenivshihsya mikroRNK i vozmozhnuyu konkurenciyu za mesta svyazyvaniya. Izmenenie ekspressii klyuchevyh genov otveta na gipoksiyu podtverdilo relevantnost' CoCl2 i proizvodnogo oksihinolina v kachestve induktorov gipoksii. Vyyavleno povyshenie urovnya mikroRNK semejstva miR-374 pri indukcii gipoksicheskogo puti v modeli trofoblasta. Nablyudaemye izmeneniya soprovozhdalis' snizheniem ekspressii mRNK gena FOXM1, kotoraya sluzhit mishen'yu dlya hsa-miR-374a-5p i hsa-miR-374b-5p. Dannye mikroRNK mogut konkurirovat' za mesta svyazyvaniya v mRNK FOXM1 s hsa-miR-21-5p. Uchastie gena FOXM1 v regulyacii invazivnogo potenciala kletok pozvolyaet predpolozhit' rol' mikroRNK miR-374 i FOXM1 v patogeneze narusheniya invazii trofoblasta pri formirovanii placenty kak predposylki k razvitiyu zaderzhki rosta ploda i preeklampsii.

Uroven' mikroRNK miR-374 povyshaetsya v kletkah BeWo b30 pri gipoksii