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Esta investigacion tuvo como objetivo principal la extraccion de ADN nuclear no invasivo de tejido branquial en los peces amazonicos Disco (Symphysodon aequifasciatus aequifasciatus) y Festivus (Mesonauta Reaccfestivus), evitando producir dano al pez o su muerte. Se logro la amplificacion molecular por Reaccion en cadena de la polimersa (PCR) y el analisis del polimorfismo genetico de los genes de color Kitlg y Tyrp1b mediante el uso de enzimas de restriccion. La amplificacion de la secuencia del gen Kitlg permitio obtener una banda de 1400 pb aproximadamente. No fue posible amplificar la secuencia del gen Tyrp1 para ninguna de las dos especies. Se identifico un sitio de corte en la secuencia del gen Kitlg en la enzima de restriccion Mse I en peces Disco de 200 a 300 pb, y una banda de aproximadamente 500 a 1000 pb con la enzima de restriccion Xho I. No se logro identificar ningun sitio de corte en la secuencia del gen Kitlg con ninguna de las enzimas de restriccion analizadas en peces Festivus. Palabras clave: Symphysodon, Mesonauta, Polimorfismo, Kitlg, Tyrp1b, peces amazonicos. Molecular amplification and identification of genetic polymorphism of color genes Tyrp1b and Kitlg in the Peruvian Amazon fish Symphysodon aequifasciatus aequifasciatus and Festivus (Mesonauta festivus) (Perciformes: Cichlidae) Abstract The goal of this research was the removal of non invasive nuclear DNA from gill tissue in Amazonian species Disco (Symphysodon aequifasciatus aequifasciatus) and Festivus (Mesonauta festivus). The PCR amplification and molecular analysis of genetic polymorphism of the color genes Tyrp1 and Kitlg using restriction enzymes was achieved. It was possible to amplify the gene sequence Kitlg (band approximately 1400 bp), but it was not possible to amplify the sequence of Tyrp1 gene for any studied species. It was identified a cleavage site in the sequence of Kitlg gene in Disco fish with the restriction enzyme Mse I from 200 to 300 bp and a band of about 500 to 1000 bp with the restriction enzyme Xho I. It was not possible to identify any cut site in the Kitlg gene sequence with any of the restriction enzymes tested in fish Festivus identified. Keywords: Symphysodon, Mesonauta, Polymorphism, Kitlg, Tyrp1b, Amazon fish.

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Amplificación e identificación molecular del polimorfismo genético de los genes de color Kitlg y Tyrp1b en los peces de la amazonía peruana Symphysodon aequifasciatus aequifasciatus y Festivus (Mesonauta festivus) (Perciformes: Cichlidae)