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This paper deals with the frequencies of albina and xantha seedlings in an M2 generation of six-rowed Pokko barley treated with sodium azide, NaN3. The dependency of these frequencies on the harvesting method of M, plants is considered. The results demonstrate that "one-grain-one-ear” harvesting in M, produced higher frequencies of both albinas and xanthas in M2 compared with an M2 originating from whole ear harvesting of M1. For albinas the difference is significant. In spite of the increased mutation rate, the laboriousness of the "one-grain-one-ear” harvesting method restricts its feasibility in practical mutation breeding.

The response of the mutation frequency of barley in M2 to the M1 harvesting method